Although many bacteria can grow in a free-living, ‘planktonic’ state, it is quite common for them to adhere to surfaces by producing extracellular polysaccharide or in some cases by means of specialised structures termed holdfasts. The adherent bacteria produce microcolonies, leading to the development of biofilms, which initially may be composed of only one bacterial type, but frequently develop to contain several bacteria living in a complex community. In fact, almost every surface exposed to liquids and nutrients will be colonised by microorganisms. A classic example is the biofilm on our teeth, leading to the development of cavities (dental caries) when bacteria such as Streptococcus mutans degrade sugars to organic acids.
Biofilms also are found on the solid supports in sewage-treatment plants, where they play an essential role in processing of sewage water before it is discharged into rivers. And biofilms are found on the surfaces of water tanks, pipes, surgical apparatus, food- processing vessels, etc., where the bacteria adhere tenaciously, resisting removal by washing and also gaining protection from the common disinfectants which cannot easily penetrate the polysaccharide matrix.

Biofilms provide interesting opportunities for experimental studies of mixed microbial communities. The image above shows part of a glass coverslip immersed for 16 hours in a nutrient solution containing two bacteria, Enterobacter agglomerans and Klebsiella pneumoniae. A plasmid containing the gene for green fluorescent protein had previously been inserted into the strain of Enterobacter, so this strain can be detected by its yellow-green fluorescence when exposed to excitation by blue light. The strain of Klebsiella was not treated but for this photograph its cells were killed and then stained with propidium iodide which gives the orange colour. We see that the two bacteria grew intimately together as a mixed microcolony. They showed greater adhesion to the glass than when they were inoculated alone, and also greater resistance to a range of antimicrobial agents in the mixed biofilm compared with single-species biofilms.

Techniques such as this can be used to ask many interesting and important questions such as:
how much adhesion is conferred by the polysaccharides of the individual species?
how does the species balance change in response to changes in environment?
is one species selectively removed from the biofilm by treatment with disinfectants?

Methane-utilising bacteria:

An interesting type of biofilm is produced by some of the highly specialised methane- oxidising bacteria (methanotrophs). These bacteria can be cultured in flasks containing a simple mineral nutrient medium, with a mixture of methane gas (CH4) as the sole carbon source and oxygen in the headspace (left-hand image above). The bacteria produce a pink-coloured, tough film resembling a sheet of polyethylene at the surface of the liquid (it has been disturbed and has become folded in the flasks shown here). The bacterial cells grow as a monolayer within this film (right-hand image), with block-like patterns of cells representing their planes of division.
Vast amounts of methane are produced in nature (in the rumen of cows, landfill sites, etc.) by anaerobic archaea (the methanogens) which gain energy by oxidation of hydrogen coupled with the reduction of carbon dioxide, to produce methane. The accumulation of methane trapped in geological deposits has produced the vast natural gas fields that are exploited as a fuel source today. In the absence of entrapment,methane diffuses into aerated sites in soil, where it is metabolised by the methylotrophs (mostly Gram negative cocci or plump rods of genera such as Methylococcus and Methylomonas). By forming a surface film at the interface between anaerobic and aerobic zones, these bacteria have a supply of methane gas (poorly soluble in water) and also a supply of oxygen essential for utilising methane. Most of these bacteria cannot use more complex organic compounds containing C-C bonds.

Kombucha tea :

Some members of the bacterial genus Acetobacter, especially Acetobacter xylinum, synthesize large amounts of cellulose when grown on sugar sources. These bacteria occur naturally on the surfaces of fruits and flowers, and have important roles in the commercial production of vinegar from wines and other fermented products. They are strictly aerobic (oxygen-requiring) organisms and they often grow after a phase of activity by fermentative organisms (e.g. yeasts), converting the fermentation end products to more oxidised forms. For example, one of their characteristic activities is to oxidise ethanol to acetic acid, so they are commonly known as acetic acid bacteria.
One of the more unusual roles of these bacteria is in the production of fermented teas, such as Kombucha tea. This tradition dates back more than 2000 years in eastern countries such as China, Japan and Russia. Recently it has become popular as a “herbal remedy” in western societies.
(see The Kombucha Network (not on this server)
The tea is made by adding a microbial culture to a mixture of black tea (cooled) and sugar, with a small amount of vinegar to acidify it. Then the mixture is incubated for 1-2 weeks. During this time, Acetobacter and various yeasts grow to produce a rubbery, pancake-like mass on the surface of the tea.



Mtech in Clinical Eng Jointly offered by Indian institute of technology Madras& Christian medical college Vellore& Sree chitra tirunal institute for medical sciences and technology Trivandrum.
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